Biological small- and wide-angle X-ray scattering beamline at the NSRRC
Orion Shih1*, Kuei-Fen Liao1, Yi-Qi Yeh1, Chun-Jen Su1, Chen-An Wang1, Je-Wei Chang1, Wei-Ru Wu1, Din-Goa Liu1, Cheng-Yuan Lin1, Cheng-Chih Liang1, Robert Lee1, Chien-Hung Chang1, Liang-Chih Chiang1, Chia-Feng Chang1, Ming-Han Lee1, Chin-Yen Liu1, Meng-Chiao Ho2, Chia-Yang Shu3, Felix Lee3, Eric Yen3, Tien-Chang Lin4, U-Ser Jeng1,4
1National Synchrotron Radiation Research Center, Hsinchu, Taiwan
2Institute of biochemical sciences & Institute of biological chemistry, Academia Sinica, Taipei, Taiwan
3Academia Sinica Grid Computing Centre, Academia Sinica, Taipei, Taiwan
4Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan
* Presenter:Orion Shih,
This presentation provides an overview of recent developments in biological small- and wide-angle X-ray scattering on the new BioSAXS beamline (TPS 13A) at the National Synchrotron Radiation Research Center. Simultaneous SAXS/WAXS from a uniquely designed detecting system comprising of an Eiger X-9M and an Eiger X-1M detector, together with an ultra-SAXS (USAXS) design for large-size detecting, the new BioSAXS beamline probes structures in a wide length scale, covering from biomolecular assemblies of tens to a hundred of nm, to global protein conformations of few nm, then down to local structural features such as cavities, tunnels, α-helix, and β-sheet of proteins/complexes in solution. An online high-performance liquid chromatography (HPLC) instrument is incorporated into the endstation for integrated measurements of SAXS/WAXS/UV-Vis absorption/Refractive-index for solution structures and compositions of biomacromolecules and their complexes. The beamline also provides opportunities to explore biomacromolecular solution structures kinetics down to microsecond time scale with the photon flux of up to 4Χ10^14 photons/s. Moreover, equipped with an optional microbeam mode, structural mapping with SAXS/WAXS in μm-resolution is possible for studies of textures in natural/synthetic bio-tissues, organelles, or biomaterials.

Keywords: BioSAXS, SEC-SAXS, biomacromolecule