Biological small- and wide-angle X-ray scattering beamline at the NSRRC

Orion Shih^1*, Kuei-Fen Liao¹, Yi-Qi Yeh¹, Chun-Jen Su¹, Chen-An Wang¹, Je-Wei Chang¹, Wei-Ru Wu¹, Din-Goa Liu¹, Cheng-Yuan Lin¹, Cheng-Chih Liang¹, Robert Lee¹, Chien-Hung Chang¹, Liang-Chih Chiang¹, Chia-Feng Chang¹, Ming-Han Lee¹, Chin-Yen Liu¹, Meng-Chiao Ho², Chia-Yang Shu³, Felix Lee³, Eric Yen³, Tien-Chang Lin⁴, U-Ser Jeng^1,4

¹National Synchrotron Radiation Research Center, Hsinchu, Taiwan
²Institute of biochemical sciences & Institute of biological chemistry, Academia Sinica, Taipei, Taiwan
³Academia Sinica Grid Computing Centre, Academia Sinica, Taipei, Taiwan
⁴Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan

* Presenter:Orion Shih, email:shih.orion@nsrrc.org.tw

This presentation provides an overview of recent developments in biological small- and wide-angle X-ray scattering on the new BioSAXS beamline (TPS 13A) at the National Synchrotron Radiation Research Center. Simultaneous SAXS/WAXS from a uniquely designed detecting system comprising of an Eiger X-9M and an Eiger X-1M detector, together with an ultra-SAXS (USAXS) design for large-size detecting, the new BioSAXS beamline probes structures in a wide length scale, covering from biomolecular assemblies of tens to a hundred of nm, to global protein conformations of few nm, then down to local structural features such as cavities, tunnels, α-helix, and β-sheet of proteins/complexes in solution. An online high-performance liquid chromatography (HPLC) instrument is incorporated into the endstation for integrated measurements of SAXS/WAXS/UV-Vis absorption/Refractive-index for solution structures and compositions of biomacromolecules and their complexes. The beamline also provides opportunities to explore biomacromolecular solution structures kinetics down to microsecond time scale with the photon flux of up to 4Χ10^14 photons/s. Moreover, equipped with an optional microbeam mode, structural mapping with SAXS/WAXS in μm-resolution is possible for studies of textures in natural/synthetic bio-tissues, organelles, or biomaterials.

Keywords: BioSAXS, SEC-SAXS, biomacromolecule